General Information

Development

Using CRISPR-Cas9 technology, the essential exon sequences of the Ldlr gene in C57BL/6NCrl mice were deleted to obtain the first-generation Ldlr knockout (KO) mice. The first-generation mice were then bred according to standard strain establishment methods to obtain homozygous Ldlr knockout mice for commercial use.

Serum cholesterol levels in Ldlr KO mice are 2 to 4 times higher than those in wild-type mice. By feeding Ldlr KO mice with a high-fat diet (HFD-fed), metabolic syndrome characterized by obesity and hypercholesterolemia, hypertriglyceridemia, and insulin resistance can be induced; these symptoms are similar to those found in human non-alcoholic steatohepatitis (NASH) models."

Phenotype

1. HE and Sirius red coloring

Ldlr KO Mice Applications

1. Diabetes and Obesity Research

2. Obesity with diabetes study

3. High-fat-induced MASH modeling

Reference

1. van Koppen A, Verschuren L, van den Hoek AM, et al. Uncovering a Predictive Molecular Signature for the Onset of NASH-Related Fibrosis in a Translational NASH Mouse Model.Cell Mol Gastroenterol Hepatol. 2017, 5(1): 83-98.